Risk factors of development of cholestatic hepatosis During pregnancy

A total of 172 pregnant women aged 15 to 47 years were examined. There was an increase in the number of patients with cholestatic hepatosis of pregnant women (HPW) from 1.1% to 1.6%. In women with HPW, hepatobiliary diseases are significantly more frequent: chronic viral hepatitis B and C (15%), chronic cholecystitis (14.2%), cholelithiasis (12.4%). HPW in 14.2% of women was in previous pregnancies. Thrombophilia, anemia and gestational hypertension can also contribute to the development of HPW.

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Central mediated gastrointestinal pain disorders: review Of main provisions of the iv roman consensus

Critical review of the main provisions of the IV Roman Consensus on central mediated gastrointestinal disorders. It is shown that the doctrine of a central mediated gastrointestinal pain disorder has undergone a significant change after the publication of the IV Roman Consensus. Causative factors of the insolvency of the regulatory relationships of the axis “brain-gastrointestinal tract” are combined morphological and physiological abnormalities associated with violations of motility of the gastrointestinal tract, mucous barrier, immune function, intestinal microbiota, visceral hypersensitivity, as well as CNS disorders. All this served as an excuse for excluding from the name of a number of disorders described by the Roman consensus the words “functional”. Also, the diagnosis of “functional abdominal pain syndrome” was changed to the syndrome of centrally induced abdominal pain (SCIAB), which is more appropriate for the pathogenesis of this disorder and reflects current information on the interaction of the gastrointestinal tract and the central nervous system. The isolation of narcotic intestinal syndrome (NIS) into a separate functional disorder, at first glance, seems illogical, since this pathology is induced by a known etiologic factor. On the other hand, the use of opioids alters the interaction between the CNS and the enteral nervous system, which may be accompanied by the development of NIS. Thus, the definition of NIS within the framework of functional pathology does not contradict the main provisions of the IV Roman Consensus of 2016.

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Comparative analysis of efficiency of simple And multicomponent methods of alkaline decellularization Through the example of cleaning of fibered extracellular Matrix of derma

Alkaline decellularization is one of the most common methods of obtaining a purified extracellular matrix (ECM). Nevertheless, there are no works in the literature devoted to a comparative analysis of the effectiveness of various methods of alkaline purification of ECM. The present work runs about a comparative study of the effect of a simple method of decellularization with sodium hydroxide solution and a multicomponent method by several different solutions based on sodium hydroxide and sodium sulfate on the biochemical, morphological and thermomechanical characteristics of the fibrous extracellular matrix of cattle dermis, namely, the Bos taurus taurus. It is shown that both methods cause effective removal of cellular components from the ECM. However, in samples purified with a multicomponent method, less residual dsDNA was detected. The results of morphological and thermomechanical studies indicate that the use of multicomponent decellularization in comparison with simple treatment with sodium hydroxide leads to a decrease in the disorganization of the ECM fibrous network, while maintaining the structure of the collagen fibers, and also to an increase in the elasticity of the material. The data obtained indicate a greater preservation of the native structure of the decellularized ECM dermis of cattle with alkaline purification using solutions containing sulfate salts. Based on the results of the work, it can be concluded that the use of a multicomponent method is more preferable for the implementation of alkaline decellularization of the cattle dermis.

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