New approaches in male infertility diagnosis: examination of biopsy material from testicle with normal and disturbed spermatogenesis

Testicle biopsy is still the most important and conclusive stage of male infertility diagnosis in the presence of azo-ospermia. Applying new advanced techniques of histological analysis allows us to evaluate spermatogenesis disturb-ance from a new angle. As a result there is a need for new seminiferous tubules signification that should use data from immune histochemical tests and be based on their phenotypic characteristics. Besides, adopted in the 1970s outdated criteria for spermatogenesis disturbance evaluation also have to be revised. As follows from the conducted study, fol-lowing classes of seminiferous epithelium should be considered: a) normal spermatogenesis; b) hypospermatogenesis; c) meiotic block to spermatogenesis; d) spermatogonial (focal case of SCO-syndrome); e) SCO-syndrome; f) tubular atrophy. Available from the tests data on spermatogenesis condition will be helpful in appropriate idiopathic male infer-tility treatment planning and in choosing the optimal assisted reproductive technology (TESE, MD-TESE, MESE, PE-SA, ICSI, IVF).

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Influence of profession and features of work on key reproductivity indicators among male patients at Florova clinic

The article runs about the impact of the nature of the activities (occupations) on the basic morphofunctional characteristics of sperm cells. Macroparameters (volume, viscosity, pH) and microparameters (motility, sperm cells morphology) were analyzed by light microscopy as well as connection between mental and physical stress on the reproductive function in men.

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Morphological characteristics of spermatogenesis in normal conditions and in idiopathic infertility (immunohistochemical aspect)

At present, the role of some biologically active substances in male idiopathic infertility is not studied well enough. Besides, little is known about the possible positive expression of oncological markers in the germ cells in idiopathic infertility. Also, there is little scientific evidence for an empirical approach in the treatment of
idiopathic infertility. Morphological analysis of normal and abnormal spermatogenesis using immunohistochemical markers (proliferation and apoptosis, growth factors, embryonic, spermatogonial, spermatocytal, locomotor apparatus) would later mark the vector of the pathogenesis and the search for adequate treatment (mostly conservative), as well as possible prevention.

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